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IAV infection increases the levels of xanthine oxidase, which is an enzyme responsible for oxygen-free radical generation in mouse serum and lung tissues, thus promoting disease pathogenesis 73

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Samples were then evaporated to dryness in Speed vac and resuspended in 500 l Buffer A (95% H 2 O, 5% CH 3 CN, 0.1% FA) with sonication and loaded on HPLC for offline high pH fractionation following conditions adapted from previous work 57